Preparation method of Pro-Pro-Val-Val

The invention belongs to the technical field of chemical analysis, and particularly relates to a preparation method of Pro-Pro-Val-Val. The preparation method of Pro-Pro-Val-Val comprises the following steps: a, extracting yellow water and an organic solvent, and concentrating an extracting solution to obtain a crude extract; b, carrying out macroporous resin column chromatography on the crude extract, collecting an eluent containing Pro-Pro-Val-Val, and concentrating to obtain a crude product; and c, carrying out gel column chromatography on the crude product, collecting an eluent containing Pro-Pro-Val-Val, and concentrating to obtain the product. According to the method, Pro-Pro-Val-Val with the purity of 95% or above is separated from yellow water with complex components for the first time, a technical basis can be provided for separation and preparation of other peptide compounds in the yellow water, and meanwhile resource utilization of the yellow water is improved. 本发明属于化学分析技术领域，具体涉及Pro-Pro-Val-Val的制备方