Protease conjugates

本发明涉及枯草杆菌蛋白酶缀合物，该缀合物包含一种蛋白酶部分和一种或多种附加部分，所述蛋白酶部分的氨基酸序列是对亲本氨基酸序列修饰后生成的序列，所述亲本氨基酸序列包含一个第一表位区域、一个第二表位区域以及一个第三表位区域，其中所述的修饰后氨基酸序列中包括用取代氨基酸在一个或多个上述表位区域中的一个或多个位置上进行的取代，其中所述的每个附加部分都与一个取代部分共价相连。本发明进一步还涉及包含此类蛋白酶缀合物的清洁及个人护理组合物。 Protease conjugates such as subtilisin conjugates are provided comprising a protease moiety and one or more addition moieties wherein the protease moiety has a modified amino acid sequence of a parent amino acid sequence. The parent amino acid sequence comprises a first epitope region, a second epitope region and a third epitope region, and the modified amino acid sequence comprises a substitution by a substituting amino acid at one or more positions in one or more of the epitope regions. When a substitution occurs in the first epitope region, the substitution occurs at one or more positions corresponding to positions 70-84 of subtilisin BPN'. When a substitution occurs in the second epitope region, the substitution occurs at one or more positions corresponding to positions 103-126 of subtilisin BPN'. When a substitution occurs in the third epitope region, the substitution occurs at one or more positions corresponding to positions 217-252 of subtilisin BPN'. Each addition moiety is covalently attached to one of the substituting amino acids. Cleaning and personal care compositions are provided containing the protease conjugates which have reduced immunogenicity relative to their corresponding parent proteases.