Immune method for improving immune protection rate of contagious pustular dermatitis virus attenuated strain

The invention provides an inoculation way suitable for an ORFV (contagious pustular dermatitis virus) ORF120 gene deleted attenuated strain through comparison of immune protection rates of a scratch inoculation mode and a subcutaneous inoculation mode. An ORFV-SY17 delta ORF120 gene deleted attenuated strain prepared by taking a self-separated ORFV-SY17 wild strain as a female parent is used for immunizing an indigenous animal lamb in a scratch and subcutaneous inoculation mode, the subcutaneous inoculation mode is safe and reliable, and local pox reaction unique to pox viruses such as papule, erythema, vesicles, pustules and scab is not generated at an inoculation part; the ORFV-SY17 delta ORF120 gene deleted attenuated strain can provide 100% immune protection for the contagious pustular dermatitis virus, and is expected to be used as an inoculation way for replacing traditional scratch immunization. 本发明提供了通过划痕、皮下两种接种方式免疫保护率的比较确定羊传染性脓疱病毒(ORFV)ORF120基因缺失减毒株适宜的接种途径。将以自行分离的ORFV-SY17野毒株为母本制备的ORFV-SY17ΔORF120基因缺