Method for evaluating acute toxicity of water body based on microorganisms and SERS (Surface Enhanced Raman Scattering) analysis

The invention discloses a method for evaluating acute toxicity of a water body based on microorganisms and SERS analysis. The method comprises the following steps: S1, activating and culturing bacteria; s2, preparing a bacterial suspension and performing toxicity incubation; s3, preparing a halogen solution; s4, performing SERS detection to obtain an indole linear graph, and correspondingly obtaining a characteristic peak value; s5, calculating the acute toxicity of the water body according to an inhibition ratio formula; the SERS method for evaluating the acute toxicity of the water body still has a relatively large development space. In addition, the detection sensitivity of acute toxicity of the water body needs to be further improved. Particularly, a new method needs to be established for detecting the acute toxicity of the water body. The invention provides an SERS method for modifying AgNPs with different halogens to improve the detection sensitivity of bacterial metabolites so as to judge the toxicity