Specific primer for identifying poecilobdella manillensis and rapid PCR (polymerase chain reaction) method

The invention discloses a specific primer for identifying poecilobdella manillensis, and the specific primer is characterized in that an upstream primer L315 is 5 '-tgtttacaggatgaacta-3', and a downstream primer H566 is 5 '-gctgctcttaatactggta-3'. The invention further discloses a kit for identifying the poecilobdella manillensis, and a kit for identifying the poecilobdella manillensis and the kit for identifying the poecilobdella manillensis. The invention relates to a rapid PCR (Polymerase Chain Reaction) method for identifying poecilobdella manillensis. A PCR reaction system comprises 12.5 mu L of Takara Premix Ex taq, 1 mu L of an upstream primer L315, 1 mu L of a downstream primer H566, 1 mu L of a DNA (Deoxyribose Nucleic Acid) template and the balance of sterile double distilled water to 25 mu L; the PCR amplification procedure is as follows: pre-denaturation at 94 DEG C for 1 min, denaturation at 94 DEG C for 30 s, annealing at 52 DEG C for 30 s, and 28 cycles. Aiming at the defects of the existing PC