Method for detecting radiation damage DNA copy number variation and application thereof

The invention discloses a method for detecting radiation damage DNA copy number variation and application thereof. The method comprises the following steps: performing radiation irradiation on extracted peripheral blood of a human body, performing sequencing and biological information analysis on a...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: DANG XUHONG, YUAN YAYI, ZHANG XIAOQUAN, DONG YUYANG, LIU HONGYAN, LIU XIAOMING, DONG JUANCONG, REN YUE, CHENG JIAO
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention discloses a method for detecting radiation damage DNA copy number variation and application thereof. The method comprises the following steps: performing radiation irradiation on extracted peripheral blood of a human body, performing sequencing and biological information analysis on a sample subjected to radiation irradiation to screen radiation damage related DNA copy number variation, and performing digital PCR verification to obtain a 14q32.33 section 464bp of radiation damage DNA copy number variation; the DNA copy number variation obtained by the method disclosed by the invention is related to chromosome aberration, and has a certain time dependency relationship and dose dependency relationship. The situation that 464bp variation of the 14q32.33 section has a dose dependency relationship is also found in accident personnel; the method is simple, the DNA copy number variation of radiation damage can be rapidly and accurately obtained, and the obtained DNA copy number variation can be used fo