Method for detecting radiation damage DNA copy number variation and application thereof
The invention discloses a method for detecting radiation damage DNA copy number variation and application thereof. The method comprises the following steps: performing radiation irradiation on extracted peripheral blood of a human body, performing sequencing and biological information analysis on a...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for detecting radiation damage DNA copy number variation and application thereof. The method comprises the following steps: performing radiation irradiation on extracted peripheral blood of a human body, performing sequencing and biological information analysis on a sample subjected to radiation irradiation to screen radiation damage related DNA copy number variation, and performing digital PCR verification to obtain a 14q32.33 section 464bp of radiation damage DNA copy number variation; the DNA copy number variation obtained by the method disclosed by the invention is related to chromosome aberration, and has a certain time dependency relationship and dose dependency relationship. The situation that 464bp variation of the 14q32.33 section has a dose dependency relationship is also found in accident personnel; the method is simple, the DNA copy number variation of radiation damage can be rapidly and accurately obtained, and the obtained DNA copy number variation can be used fo |
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