Kit for rapidly identifying plasmodium falciparum based on RPA-CRISPR/Cas12a fluorescent system
The invention discloses a kit for rapidly identifying plasmodium falciparum based on an RPA-CRISPR/Cas12a fluorescent system, and belongs to the field of detection and inspection. The kit comprises a plasmodium falciparum RPA specific primer pair, a crRNA fragment, a fluorescent report probe, a buff...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | LIN MIN WEI HUAGUI WANG CHUNFANG CHEN LIYING HUANG WEIYI WANG JUNLI ZHENG YUZHONG ZHONG TIANYU NONG LEGEN |
| description | The invention discloses a kit for rapidly identifying plasmodium falciparum based on an RPA-CRISPR/Cas12a fluorescent system, and belongs to the field of detection and inspection. The kit comprises a plasmodium falciparum RPA specific primer pair, a crRNA fragment, a fluorescent report probe, a buffer solution for CRISPR (clustered regularly interspaced short palindromic repeats) reaction and Cas12a protein. Based on an RPA technology, a CRISPR/Cas12a technology and a fluorescence detection technology, the kit and the detection method which are high in sensitivity, high in specificity, simple in operation method, rapid and accurate in detection and identification of plasmodium falciparum are constructed. The constructed kit and the detection method can be used for detecting the plasmodium falciparum in a field or clinical environment, particularly in countries with limited resources, and the detection method does not need expensive instruments and equipment and professional standard laboratories, and has impo |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN116334186A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN116334186A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN116334186A3</originalsourceid><addsrcrecordid>eNqNjEEKwjAQRbtxIeodxgMUiRVxW4KiCFKq-zI2ExlIm5BJF729WXgAV-8v3n_LortzAusjRAxs3AxsaExsZx4_EBzK4A1PA1h0PQeMeb5RyIAfoW3qUre3Z9PuNIraI1g3-UjS5wTILImGdbHIV6HNj6tiezm_9LWk4DuSgNml1OmHUseqOqjTsa7-cb4QMjv9</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Kit for rapidly identifying plasmodium falciparum based on RPA-CRISPR/Cas12a fluorescent system</title><source>esp@cenet</source><creator>LIN MIN ; WEI HUAGUI ; WANG CHUNFANG ; CHEN LIYING ; HUANG WEIYI ; WANG JUNLI ; ZHENG YUZHONG ; ZHONG TIANYU ; NONG LEGEN</creator><creatorcontrib>LIN MIN ; WEI HUAGUI ; WANG CHUNFANG ; CHEN LIYING ; HUANG WEIYI ; WANG JUNLI ; ZHENG YUZHONG ; ZHONG TIANYU ; NONG LEGEN</creatorcontrib><description>The invention discloses a kit for rapidly identifying plasmodium falciparum based on an RPA-CRISPR/Cas12a fluorescent system, and belongs to the field of detection and inspection. The kit comprises a plasmodium falciparum RPA specific primer pair, a crRNA fragment, a fluorescent report probe, a buffer solution for CRISPR (clustered regularly interspaced short palindromic repeats) reaction and Cas12a protein. Based on an RPA technology, a CRISPR/Cas12a technology and a fluorescence detection technology, the kit and the detection method which are high in sensitivity, high in specificity, simple in operation method, rapid and accurate in detection and identification of plasmodium falciparum are constructed. The constructed kit and the detection method can be used for detecting the plasmodium falciparum in a field or clinical environment, particularly in countries with limited resources, and the detection method does not need expensive instruments and equipment and professional standard laboratories, and has impo</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230627&DB=EPODOC&CC=CN&NR=116334186A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25551,76302</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230627&DB=EPODOC&CC=CN&NR=116334186A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIN MIN</creatorcontrib><creatorcontrib>WEI HUAGUI</creatorcontrib><creatorcontrib>WANG CHUNFANG</creatorcontrib><creatorcontrib>CHEN LIYING</creatorcontrib><creatorcontrib>HUANG WEIYI</creatorcontrib><creatorcontrib>WANG JUNLI</creatorcontrib><creatorcontrib>ZHENG YUZHONG</creatorcontrib><creatorcontrib>ZHONG TIANYU</creatorcontrib><creatorcontrib>NONG LEGEN</creatorcontrib><title>Kit for rapidly identifying plasmodium falciparum based on RPA-CRISPR/Cas12a fluorescent system</title><description>The invention discloses a kit for rapidly identifying plasmodium falciparum based on an RPA-CRISPR/Cas12a fluorescent system, and belongs to the field of detection and inspection. The kit comprises a plasmodium falciparum RPA specific primer pair, a crRNA fragment, a fluorescent report probe, a buffer solution for CRISPR (clustered regularly interspaced short palindromic repeats) reaction and Cas12a protein. Based on an RPA technology, a CRISPR/Cas12a technology and a fluorescence detection technology, the kit and the detection method which are high in sensitivity, high in specificity, simple in operation method, rapid and accurate in detection and identification of plasmodium falciparum are constructed. The constructed kit and the detection method can be used for detecting the plasmodium falciparum in a field or clinical environment, particularly in countries with limited resources, and the detection method does not need expensive instruments and equipment and professional standard laboratories, and has impo</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjEEKwjAQRbtxIeodxgMUiRVxW4KiCFKq-zI2ExlIm5BJF729WXgAV-8v3n_LortzAusjRAxs3AxsaExsZx4_EBzK4A1PA1h0PQeMeb5RyIAfoW3qUre3Z9PuNIraI1g3-UjS5wTILImGdbHIV6HNj6tiezm_9LWk4DuSgNml1OmHUseqOqjTsa7-cb4QMjv9</recordid><startdate>20230627</startdate><enddate>20230627</enddate><creator>LIN MIN</creator><creator>WEI HUAGUI</creator><creator>WANG CHUNFANG</creator><creator>CHEN LIYING</creator><creator>HUANG WEIYI</creator><creator>WANG JUNLI</creator><creator>ZHENG YUZHONG</creator><creator>ZHONG TIANYU</creator><creator>NONG LEGEN</creator><scope>EVB</scope></search><sort><creationdate>20230627</creationdate><title>Kit for rapidly identifying plasmodium falciparum based on RPA-CRISPR/Cas12a fluorescent system</title><author>LIN MIN ; WEI HUAGUI ; WANG CHUNFANG ; CHEN LIYING ; HUANG WEIYI ; WANG JUNLI ; ZHENG YUZHONG ; ZHONG TIANYU ; NONG LEGEN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116334186A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIN MIN</creatorcontrib><creatorcontrib>WEI HUAGUI</creatorcontrib><creatorcontrib>WANG CHUNFANG</creatorcontrib><creatorcontrib>CHEN LIYING</creatorcontrib><creatorcontrib>HUANG WEIYI</creatorcontrib><creatorcontrib>WANG JUNLI</creatorcontrib><creatorcontrib>ZHENG YUZHONG</creatorcontrib><creatorcontrib>ZHONG TIANYU</creatorcontrib><creatorcontrib>NONG LEGEN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIN MIN</au><au>WEI HUAGUI</au><au>WANG CHUNFANG</au><au>CHEN LIYING</au><au>HUANG WEIYI</au><au>WANG JUNLI</au><au>ZHENG YUZHONG</au><au>ZHONG TIANYU</au><au>NONG LEGEN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Kit for rapidly identifying plasmodium falciparum based on RPA-CRISPR/Cas12a fluorescent system</title><date>2023-06-27</date><risdate>2023</risdate><abstract>The invention discloses a kit for rapidly identifying plasmodium falciparum based on an RPA-CRISPR/Cas12a fluorescent system, and belongs to the field of detection and inspection. The kit comprises a plasmodium falciparum RPA specific primer pair, a crRNA fragment, a fluorescent report probe, a buffer solution for CRISPR (clustered regularly interspaced short palindromic repeats) reaction and Cas12a protein. Based on an RPA technology, a CRISPR/Cas12a technology and a fluorescence detection technology, the kit and the detection method which are high in sensitivity, high in specificity, simple in operation method, rapid and accurate in detection and identification of plasmodium falciparum are constructed. The constructed kit and the detection method can be used for detecting the plasmodium falciparum in a field or clinical environment, particularly in countries with limited resources, and the detection method does not need expensive instruments and equipment and professional standard laboratories, and has impo</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN116334186A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Kit for rapidly identifying plasmodium falciparum based on RPA-CRISPR/Cas12a fluorescent system |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T20%3A49%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=LIN%20MIN&rft.date=2023-06-27&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN116334186A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |