Kit for rapidly identifying plasmodium falciparum based on RPA-CRISPR/Cas12a fluorescent system
The invention discloses a kit for rapidly identifying plasmodium falciparum based on an RPA-CRISPR/Cas12a fluorescent system, and belongs to the field of detection and inspection. The kit comprises a plasmodium falciparum RPA specific primer pair, a crRNA fragment, a fluorescent report probe, a buff...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a kit for rapidly identifying plasmodium falciparum based on an RPA-CRISPR/Cas12a fluorescent system, and belongs to the field of detection and inspection. The kit comprises a plasmodium falciparum RPA specific primer pair, a crRNA fragment, a fluorescent report probe, a buffer solution for CRISPR (clustered regularly interspaced short palindromic repeats) reaction and Cas12a protein. Based on an RPA technology, a CRISPR/Cas12a technology and a fluorescence detection technology, the kit and the detection method which are high in sensitivity, high in specificity, simple in operation method, rapid and accurate in detection and identification of plasmodium falciparum are constructed. The constructed kit and the detection method can be used for detecting the plasmodium falciparum in a field or clinical environment, particularly in countries with limited resources, and the detection method does not need expensive instruments and equipment and professional standard laboratories, and has impo |
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