Optimized lentiviral transfer vectors and uses thereof

The present invention relates to a lentiviral transfer vector comprising a heterologous nucleic acid to be introduced into a cell. The lentiviral transfer vector can be characterized by the following features: (a) comprising a cytomegalovirus (CMV) promoter; (b) a polynucleotide encoding a partial gag protein comprising a mutant INS1 inhibition sequence that reduces RNA nuclear output restriction; (c) a polynucleotide that does not comprise INS2, INS3, and INS4 inhibitory sequences encoding gag; (d) no SV40 replication starting point and/or f1 replication starting point is included; (e) a cPPT sequence containing a splicing site; (f) comprising an EF1 [alpha] promoter with intact splice donor and receptor sites; and (g) a hepatitis B PRE comprising a mutation in the initiation codon of the X protein ORF. 本发明涉及包含待引入细胞的异源核酸的慢病毒转移载体。该慢病毒转移载体可以表征为以下特征：(a)包含巨细胞病毒(CMV)启动子；(b)包含含有减少RNA核输出限制的突变INS1抑制序列的编码部分gag蛋白质的多核苷酸；(c)不包含编码gag的INS2、INS3和INS4抑制序列的多核苷酸；(d)不包含SV40复制起点和/或f1复制起点；(e)包含含有剪接位点的cPPT序列；(f)包含具有完整剪接供体和受体位点的EF