Double sgRNA of targeted Was gene, mESC cell line of knockout Was gene and application of mESC cell line

The invention belongs to the technical field of gene engineering, and particularly relates to double sgRNA of a targeted Was gene, an mESC cell line of a knockout Was gene and application of the mESC cell line. The double sgRNA of the targeted Was gene comprises a Was-sgRNA (Ribonucleic Acid) 1 and a Was-sgRNA 2, and the double sgRNA of the targeted Was gene comprises the Was-sgRNA 1 and the The target sequence of the Was-sgRNA1 is CACAGTTGTTCAGCTACC, and the target sequence of the Was-sgRNA1 is CACAGTTGTTCAGCTACC. The target sequence of the Was-sgRNA2 is GGATGAAGTAGGACTTCTGA, and the target sequence of the Was-sgRNA2 is shown in the specification. According to the invention, two sgRNA sequences targeting the second exon of the mouse Was gene respectively are adopted, a large-fragment gene knockout cell line can be directly obtained through one-time cell screening, the knockout effect is more thorough, and the severe phenotype of the WAS syndrome can be better simulated. 本发明属于基因工程技术领域，具体涉及靶向Was基因的双sgRNA和敲除Was