Recombinant Getavirus for expressing Japanese encephalitis virus E protein and construction method and vaccine application thereof

The invention discloses a recombinant Getavirus for expressing Japanese encephalitis virus E protein, the virus has a Japanese encephalitis virus E protein gene, and the E protein gene is located between Cap and E3 genes of a Getavirus genome. A corresponding construction method is also established, the Geitavirus is used as a parent strain, the infectious clone pGETV-GV of the Geitavirus is used as a carrier, and a truncated Japanese encephalitis virus E protein is inserted between Cap and E3 by using a genetic engineering method to obtain the recombinant Geitavirus rGECJEV-E. Tests show that the recombinant Geitavirus rGECJEV-E has the characteristics of good characteristics, good stability and high foreign protein expression quantity, and the expressed protein has good immunogenicity and can provide immune protection for attack of the Japanese encephalitis virus; and the recombinant strain is relatively high in safety. Therefore, the recombinant virus has great potential in preparation of vaccines or medic