Construction and identification method of transgenic fruit fly model with abnormal intestinal acidity

Construction and identification method of transgenic fruit fly model with abnormal intestinal acidity

The invention relates to a construction and identification method of transgenic drosophila melanogaster capable of inducing intestinal acidity abnormality, a drosophila melanogaster transgenic vector pValium 20-RagA RNAi is constructed, a RagA RNAi transgenic drosophila melanogaster is obtained by a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: ZHOU YING, NIU CHUNMEI, GE CHURUI, WEI YOUHENG
Format: Patent
Sprache:chi ; eng
Schlagworte:
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CARE OF BIRDS, FISHES, INSECTS
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW BREEDS OF ANIMALS
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR
SPIRITS
TESTING
TRAPPING
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention relates to a construction and identification method of transgenic drosophila melanogaster capable of inducing intestinal acidity abnormality, a drosophila melanogaster transgenic vector pValium 20-RagA RNAi is constructed, a RagA RNAi transgenic drosophila melanogaster is obtained by a microinjection method, and the RagA RNAi transgenic drosophila melanogaster is subjected to a two-balance drosophila melanogaster SP/SM6 (double balance); hybridizing MKRS/TM6 for two generations to obtain UAS-RagA RNAi transgenic drosophila melanogaster SP/SM6 which has a balancer gene and can be stably inherited; the UAS-RagA RNAi/TM6 is hybridized with NP1-GAL4 drosophila melanogaster which drives gene expression in intestinal epithelial cells, so that NP1-GAL4 of which RagA is knocked down in intestinal tracts is obtained; the invention relates to UAS-RagA RNAi (Ribonucleic Acid Interference) Phenotype detection shows that the intestinal morphology of the drosophila melanogaster is abnormal; a method for feedi