Production of oligosaccharide mixtures by means of cells

The invention belongs to the technical field of synthetic biology and metabolic engineering transformation. More specifically, the invention is in the technical field of culture or fermentation of metabolically engineered cells. A cell that has been metabolically engineered for use in the manufacture of a mixture of at least three different oligosaccharides is described. In addition, the present invention provides a method for producing a mixture of at least three different oligosaccharides by means of cells and for purifying at least one of said oligosaccharides from culture. 本发明处于合成生物学及代谢工程改造的技术领域。更特定言之，本发明处于经代谢工程改造的细胞的培养或发酵的技术领域。本发明描述一种经代谢工程改造以用于制造至少三种不同寡糖的混合物的细胞。此外，本发明提供一种用于借由细胞制造至少三种不同寡糖的混合物以及自培养纯化所述寡糖中的至少一者的方法。