Multi-targeting bispecific antigen binding molecules with increased selectivity

The present invention provides a multi-targeting bispecific antigen-binding molecule characterized by comprising a first and a second bispecific entity, each entity comprising a domain that binds to a target, a second domain that binds to an extracellular epitope of human and macaque CD3 epsilon chains wherein the two bispecific entities are linked to each other by a spacer, the spacer separates the first and second bispecific entities. In addition, the invention provides polynucleotides encoding the multi-targeting bispecific antigen binding molecules, vectors comprising such polynucleotides, host cells expressing constructs, and pharmaceutical compositions comprising the same. 本发明提供了多靶向性双特异性抗原结合分子，其特征在于包含第一和第二双特异性实体，每个实体包含与靶标结合的结构域、与人和猕猴CD3ε链的细胞外表位结合的第二结构域，其中两个双特异性实体通过间隔物彼此连接，该间隔物将第一和第二双特异性实体间隔开。此外，本发明提供了编码该多靶向性双特异性抗原结合分子的多核苷酸、包含这种多核苷酸的载体、表达构建体的宿主细胞、以及包含其的药物组合物。