Method for establishing fingerprint spectrum of gynostemma pentaphyllum serving as raw medicinal material of nine-ingredient blood replenishing oral liquid

The invention discloses a method for establishing a fingerprint spectrum of a raw medicinal material gynostemma pentaphylla of nine-ingredient blood-enriching oral liquid, and relates to the technical field of medicine quality analysis, and the key point of the technical scheme is that the method specifically comprises the following steps: S1, preparation of a test sample: precisely weighing 1g of a gynostemma pentaphylla medicinal material, and placing the gynostemma pentaphylla medicinal material in a conical flask with a plug; then precisely adding 20ml of 70% ethanol, weighing, heating and refluxing for 30 minutes, taking out, and cooling; and supplementing the lost weight with 70% ethanol, uniformly shaking, and filtering to obtain the traditional Chinese medicine composition. S2, specific chromatogram detection: sucking 10 microliters of the test solution prepared in the step S1, and injecting the test solution into a liquid chromatograph; the method comprises the following steps: taking octadecyl silic