Colorimetric detection method for glucose and hydrogen peroxide by using magnetic nitrogen-doped carbon chitin microsphere nano-enzyme

The invention belongs to the technical field of detection sensor materials, and particularly relates to a colorimetric detection method of magnetic nitrogen-doped carbon chitin microsphere nano-enzyme on glucose and hydrogen peroxide. Glucose oxidase is utilized to oxidize glucose to generate hydrog...

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Hauptverfasser: TIAN JIANWEN, DAI JIANGBEI, LIU YUE, PENG HAILONG, KUANG JIE, CAI TAIMEI, LIU FEIFAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of detection sensor materials, and particularly relates to a colorimetric detection method of magnetic nitrogen-doped carbon chitin microsphere nano-enzyme on glucose and hydrogen peroxide. Glucose oxidase is utilized to oxidize glucose to generate hydrogen peroxide, and then magnetic nitrogen-doped carbon chitin microsphere nano-enzyme is used as a catalyst to catalyze a chromogenic reaction of the hydrogen peroxide and a color developing agent to detect glucose and H2O2, so that the problems of high operation requirements, complex detection process, high detection cost, long detection time, high detection efficiency and the like of glucose detection in the prior art can be solved. The method disclosed by the invention is low in cost and simple and convenient to operate, and the content of glucose and H2O2 in a sample can be visually and rapidly detected. 本发明属于检测传感器材料的技术领域,具体涉及一种磁性氮掺杂碳甲壳素微球纳米酶对葡萄糖和过氧化氢的比色检测方法。利用葡萄糖氧化酶氧化葡萄糖生成过氧化氢,再以磁性氮掺杂碳甲壳素微球纳米酶为催化剂催化过氧化氢与显色剂的显色反应