Method for rapidly detecting organophosphorus pesticide by using nano-enzyme-based 3D printing colorimetric device

The invention discloses a method for rapidly detecting an organophosphorus pesticide by using a nano-enzyme-based 3D printing colorimetric device. According to the invention, a Cu-C3N4 nano material with peroxidase (POD)-like properties is synthesized, and the Cu-C3N4 nano material, acetylcholin esterase and choline oxidase jointly construct a freeze-dried biological capsule. The acetylcholine can react with acetylcholin esterase and choline oxidase to generate H2O2, and the H2O2 can oxidize 3, 3, 5, 5-tetramethyl benzidine (TMB) under the catalysis of Cu-C3N4, so that the solution becomes blue. The existence of paraoxon can inhibit the activity of acetylcholin esterase so as to inhibit the color change. A 3D printing colorimetric device based on a smart phone is used for recording a color digital image and converting the color digital image into a gray value, and the oxygen and phosphorus pesticide concentration is obtained through the intensity of the gray value. According to the method, the detection metho