RT-LAMP detection primer and kit for hippeastrum chlorotic ringspot virus as well as application and method of RT-LAMP detection primer and kit

The invention relates to hippeastrum chlorotic ringspot virus RT-LAMP (reverse transcription loop-mediated isothermal amplification) detection primers, a kit as well as application and a method thereof, belonging to the technical field of molecular biology. The RT-LAMP detection primer group for the hippeastrum chlorotic ringspot virus comprises an outer primer pair HCRV-NP-F3-1 and an outer primer pair HCRV-NP-B3-1, wherein the sequences of the outer primer pair HCRV-NP-F3-1 and the outer primer pair HCRV-NP-B3-1 are shown as SEQ ID NO.1 and SEQ ID NO.2; an inner primer pair HCRV-NP-FIP-1 and an inner primer pair HCRV-NP-BIP-1, the sequences of which are as shown in SEQ ID NO.3 and SEQ ID NO.4; the kit comprises a loop primer pair HCRV-NP-LF-1 and a loop primer pair HCRV-NP-LB-1, wherein the sequences of the loop primer pair HCRV-NP-LF-1 and the loop primer pair HCRV-NP-LB-1 are shown as SEQ ID The sensitivity of the primer group for detecting the HCRV is 100 times that of conventional RT-PCR (Reverse Transc