Primer and probe combination for detecting single nucleotide polymorphism and detection method

The invention discloses a primer and probe combination for detecting single nucleotide polymorphism and a detection method. The primer and probe combination comprises a primer and a probe which are used for detecting the UGT1A1 * 6 site and the UGT1A1 * 28 site of the UGT1A1 gene. According to the fluorescent quantitative PCR detection technology based on the fluorescent chain melting curve, the specific amplification primer and the probe are ingeniously designed, after fluorescent quantitative PCR amplification is carried out in a one-tube reaction system, the result can be interpreted through the melting curve peak shape and the Tm value, operation after PCR is not needed, the detection steps are simple and convenient, the detection period is short, and the detection time is short. Detection can be completed by only 2 h for removing sample extraction, uncovering operation is avoided, cross contamination of all samples is avoided, the cost is low, meanwhile, high-sensitivity and accurate detection can be ach