Construction of PPAR gamma knockout cell line and application of PPAR gamma knockout cell line as PRRSV (porcine reproductive and respiratory syndrome virus) production cell line

The invention belongs to the technical field of antiviral research, and particularly relates to construction of a PPAR gamma knockout cell line and application of the PPAR gamma knockout cell line as a PRRSV (porcine reproductive and respiratory syndrome virus) production cell line. Firstly, the invention accidentally finds that the replication of the PRRSV can be obviously promoted by knocking out the PPAR gamma gene in the host cell, so that the cell line constructed by inhibiting/interfering/silencing/knocking out the PPAR gamma gene in the host cell can be used as a PRRSV or PRRSV vaccine production cell line to promote the production of the PRRSV or PRRSV vaccine; secondly, the PPAR gamma gene can be used as a target spot for screening drugs for preventing or treating PRRS, is used for developing drugs for preventing and treating PRRSV and researching anti-PRRSV transgenic pigs, and has a wide application prospect. 本发明属于抗病毒研究技术领域，具体涉及一种PPARγ敲除细胞系的构建及作为PRRSV生产细胞系的应用。首先，本发明意外发现，在宿主细胞中敲除PPARγ基因，能够显著促进PRRSV的