Extraction method of DNA (Deoxyribonucleic Acid) of sugarcane leaves

The invention discloses a method for extracting DNA (deoxyribonucleic acid) of sugarcane leaves. The method comprises the following steps: adding stainless steel beads into a centrifugal tube filled with the sugarcane leaves; taking out grids of a centrifugal tube rack matched with the centrifugal tubes in size, placing the centrifugal tubes in the grids, then placing the grids in liquid nitrogen for treatment, and shaking up and down until the sugarcane leaves are crushed; and adding the crude extraction solution, carrying out metal bath, and centrifuging to obtain the supernatant. The extraction method of the DNA of the sugarcane leaves is simple, efficient, time-saving and labor-saving, the extracted DNA can be directly used for PCR amplification, and amplification bands are clear. 本发明公开了一种甘蔗叶片DNA的提取方法，其是在装有甘蔗叶片的离心管中加入不锈钢珠；取出与离心管大小匹配的离心管架的网格，将离心管置于所述网格中，再将所述网格置于液氮中处理后，上下晃动直至甘蔗叶片粉碎；再加入粗提溶液后进行金属浴，离心取上清，即得。本发明的甘蔗叶片DNA的提取方法简单、高效、省时省力，且提取的DNA可以直接用于PCR扩增，扩增条带清晰。