Preparation method and application of biochar phosphate-solubilizing bacterium complex microbial inoculant

The invention discloses a preparation method and application of a biochar and phosphate-solubilizing bacterium complex microbial inoculant, and belongs to the technical field of microorganisms. The method comprises the following steps: (1) preparing biochar; (2) activating strains; (3) amplification culture: transferring the activated strain to an inorganic phosphorus liquid culture medium, culturing in a constant-temperature shaking table, and taking out; (4) attachment culture: transferring the bacterial liquid subjected to amplification culture into an LB culture solution, adding charcoal, and culturing in a constant-temperature incubator; and (5) adding biochar for attachment culture, centrifuging, and removing superfluous culture medium on the upper layer to obtain the biochar phosphate-solubilizing bacterium complex microbial inoculant. The preparation method of the biochar phosphate-solubilizing bacterium complex microbial inoculant mainly comprises the steps of biochar preparation, microorganism cultu