Recombinant type I collagenase and preparation method and application of mutant of recombinant type I collagenase

The invention discloses a recombinant I-type collagenase and a preparation method and application of a mutant of the recombinant I-type collagenase. The I-type collagenase mutant provided by the invention has relatively good alkali resistance and can be suitable for extracting fish skin waste by an enzymolysis method; the invention also provides a prokaryotic expression system-based recombinant I-type collagenase and an expression method of the mutant thereof, the expression quantity of soluble protein in the method is improved, the product activity is high, and the method is suitable for industrial large-scale production. 本申请公开了一种重组I型胶原蛋白酶及其突变体的制备方法和应用。本申请中提供了I型胶原蛋白酶突变体，其具有较好的耐碱性，可适用于酶解法提取鱼皮废料；本申请还提供了基于原核表达体系的重组I型胶原蛋白酶及其突变体的表达方法，该方法可溶蛋白表达量提高，并且产物活性高，适宜工业化放大生产。