Preparation method of aquatic plant cauliflower leaf protoplast and kit

The invention provides a preparation method of leaf protoplast of water cauliflower and a kit. According to the method, leaves of the water cauliflower are used as materials, and a water cauliflower leaf protoplast preparation system is established by adjusting an enzymolysis system, a washing system and a purification method. 1% of cellulase, 0.5% of pectinase and 0.5% of macerozyme in an enzymatic hydrolysate system have a good enzymolysis effect, MES, sorbitol and KCl are compounded to ensure that the osmotic potential of an external medium is the same as that of cells when the protoplast is dissociated, the activity of the protoplast is maintained, and the osmotic pressure of the protoplast can be well maintained to be stable by adopting a 26% sucrose solution; according to the method, the protoplast cells of the leaf tissues of the cauliflower can be rapidly and efficiently dissociated, and the separated protoplast is large in quantity, high in activity and pure in background. The result can be directly