Culture method for reducing acid peak proportion of antibody protein, antibody and cell culture medium

The invention discloses a culture method for reducing the acid peak proportion of antibody-like proteins, an antibody and a cell culture medium. The culture method comprises the following steps: inoculating fusion cells into a basic culture medium for culture, and adding a fed-batch culture medium and a regulator in the culture process; separating the culture product to obtain an antibody protein; wherein the regulator comprises Mn < 2 + >. After the regulator is added, the molar concentration of Mn < 2 + > in the culture system is 20-30 [mu] M. According to the culture method, the acid peak proportion of antibody protein can be effectively reduced, the influence on an alkali peak is low, and compared with an existing culture method, the acid peak proportion of the antibody is reduced by 29.2%, and the alkali peak proportion is only increased by 4.5%; the method does not need to change process control conditions, has no influence on cell growth and target antibody yield, and does not cause other regulations a