Chimeric antigen receptor capable of specifically recognizing Claudin18.2 and CAR-NK cell applying same

The invention provides a chimeric antigen receptor capable of specifically recognizing Claudin18.2 and a CAR-NK (Chimeric Antigen Receptor-Natural Killer) cell applying the chimeric antigen receptor. The chimeric antigen receptor comprises a single-chain antibody capable of specifically recognizing Claudin18.2, a transmembrane structural domain and an intracellular structural domain which are sequentially connected in series, the amino acid sequence of the single-chain antibody capable of specifically recognizing Claudin18.2 is as shown in SEQ ID: 1. The chimeric antigen receptor capable of specifically recognizing Claudin18.2, provided by the invention, can be used for effectively activating immune functional cells and enabling the immune functional cells to efficiently kill ovarian cancer cells and gastric cancer cells. 本发明提供一种可特异性识别Claudin18.2的嵌合抗原受体及应用其的CAR-NK细胞。该嵌合抗原受体包括依次串联的可特异性识别Claudin18.2的单链抗体、跨膜结构域和胞内结构域；可特异性识别Claudin18.2的单链抗体的氨基酸序列如SEQ ID：1所示。本发明提供的可特异性识别Claudin18.2的嵌合抗原受体能够有效激活免疫功能细胞并使免疫功能细胞高效地杀卵巢