Chimeric TsCas12a protein as well as preparation method and application thereof

The invention relates to a chimera TsCas12a protein as well as a preparation method and application thereof, and belongs to the technical field of molecular biology. The chimera TsCas12a protein comprises an active region which is formed by sequentially connecting functional fragments of amino acid sequences as shown in SEQ ID NO.1-SEQ ID NO.11. The chimera TsCas12a protein is a chimera TsCas12a protein. The chimera TsCas12a protein can realize high-sensitivity and high-precision molecular detection in a system in which high-concentration PEG (polyethylene glycol), a nonionic detergent and a reducing agent exist. Not only is a new choice of a necessary tool provided for Cas12a-based nucleic acid molecule detection, but also development of a combination scheme of the novel CRISPR-TsCas12a system and other isothermal amplification one-step methods is facilitated. 本发明涉及一种嵌合体TsCas12a蛋白及制备方法和应用，属于分子生物学技术领域。该嵌合体TsCas12a蛋白包含由SEQ ID NO.1-SEQ ID NO.11所示氨基酸序列的功能片段依次连接组成的活性区域。该嵌合体TsCas12a蛋白可在高浓度PEG、非离子型去污剂和还原剂存在的体系中实现高灵