Variant pseudorabies virus gC-gD epitope concatemer and application thereof

The invention provides a gC-gD epitope concatemer of a mutant pseudorabies virus (PRV) and an application of the gC-gD epitope concatemer of the mutant pseudorabies virus (PRV). The amino acid sequence of the mutant pseudorabies virus gC-gD expression concatemer is as shown in SEQ ID NO.1, and the n...

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Bibliographische Detailangaben
Hauptverfasser:	WU XUEMIN, LIU YUTAO, CHE YONGLIANG, YAN SHAN, CHEN RUJING, WANG LONGBAI, CHEN QIUYONG, ZHOU LUNJIANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a gC-gD epitope concatemer of a mutant pseudorabies virus (PRV) and an application of the gC-gD epitope concatemer of the mutant pseudorabies virus (PRV). The amino acid sequence of the mutant pseudorabies virus gC-gD expression concatemer is as shown in SEQ ID NO.1, and the nucleotide sequence of the coding gene of the mutant pseudorabies virus gC-gD expression concatemer is as shown in SEQ ID NO.2. The variant pseudorabies virus gC-gD epitope concatemer is utilized to establish a PRV neutralizing antibody indirect ELISA detection method with strong specificity, high sensitivity and good repeatability, serological diagnosis requirements of pig farms with PRV wild virus prevalence and pig herds in PRV wild virus purification pig farms can be completely met, and a certain foundation is laid for development of novel PRV polypeptide vaccines. 本发明提供了一种变异伪狂犬病病毒（Pseudorabies virus，PRV）gC-gD表位串联体及其应用。所述变异伪狂犬病病毒gC-gD表达串联体的氨基酸序列如SEQ ID NO.1所示，其编码基因的核苷酸序列如SEQ ID NO.2所示。本发明利用该变异伪狂犬病病毒gC-gD表位串联体建立了