Novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as preparation method and application of B-cell immunodominance epitope peptide
The invention discloses a novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as a preparation method and application of the B-cell immunodominance epitope peptide, and belongs to the technical field of coronavirus detection. The method comprises the following st...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | CHEN YING ZHANG FANG WU CHAO WANG QINJIN NING JIE LI BIN YE KAIJIE ZHAI AIXIA HE TAOJUN CHEN XINGCHI |
| description | The invention discloses a novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as a preparation method and application of the B-cell immunodominance epitope peptide, and belongs to the technical field of coronavirus detection. The method comprises the following steps: detecting and screening new coronavirus nucleocapsid protein specific IgG and IgM antibody positive serum samples by adopting an indirect ELISA method, overlapping the new coronavirus nucleocapsid protein to synthesize 68 short peptides, synthesizing 8 peptide libraries from the 68 short peptides, and screening to obtain an IgG and IgM immunodominance peptide library of the new coronavirus nucleocapsid protein. And finally, screening out the immunodominance epitope peptide with the sequence as shown in SEQ ID NO.66 again. The immunodominance epitope peptide does not contain unnecessary parts, can be used for serological detection and supplement of vaccine effect evaluation, and has great vaccine application va |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN116284267A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN116284267A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN116284267A3</originalsourceid><addsrcrecordid>eNqNjTEKwkAQRdNYiHqH8QApEiXaalCsUtmHYTPBgc3MsLuJV_KYRvQAFp8Hn8_7y-zV6EQezrkj74GHYRTtdGBBcQRknNQIjCxxR6A9OA0qOHEYI8joPKlDi9yBBU3EAhjh-XHNtECGAROrwEDpoR2gzDHz7L71bPzve50tevSRNj-usu31cq9vOZm2FA0dCaW2boqiKo_7sjqcdv9s3sRUWSw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as preparation method and application of B-cell immunodominance epitope peptide</title><source>esp@cenet</source><creator>CHEN YING ; ZHANG FANG ; WU CHAO ; WANG QINJIN ; NING JIE ; LI BIN ; YE KAIJIE ; ZHAI AIXIA ; HE TAOJUN ; CHEN XINGCHI</creator><creatorcontrib>CHEN YING ; ZHANG FANG ; WU CHAO ; WANG QINJIN ; NING JIE ; LI BIN ; YE KAIJIE ; ZHAI AIXIA ; HE TAOJUN ; CHEN XINGCHI</creatorcontrib><description>The invention discloses a novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as a preparation method and application of the B-cell immunodominance epitope peptide, and belongs to the technical field of coronavirus detection. The method comprises the following steps: detecting and screening new coronavirus nucleocapsid protein specific IgG and IgM antibody positive serum samples by adopting an indirect ELISA method, overlapping the new coronavirus nucleocapsid protein to synthesize 68 short peptides, synthesizing 8 peptide libraries from the 68 short peptides, and screening to obtain an IgG and IgM immunodominance peptide library of the new coronavirus nucleocapsid protein. And finally, screening out the immunodominance epitope peptide with the sequence as shown in SEQ ID NO.66 again. The immunodominance epitope peptide does not contain unnecessary parts, can be used for serological detection and supplement of vaccine effect evaluation, and has great vaccine application va</description><language>chi ; eng</language><subject>CHEMISTRY ; HUMAN NECESSITIES ; HYGIENE ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; TESTING</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230623&DB=EPODOC&CC=CN&NR=116284267A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230623&DB=EPODOC&CC=CN&NR=116284267A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHEN YING</creatorcontrib><creatorcontrib>ZHANG FANG</creatorcontrib><creatorcontrib>WU CHAO</creatorcontrib><creatorcontrib>WANG QINJIN</creatorcontrib><creatorcontrib>NING JIE</creatorcontrib><creatorcontrib>LI BIN</creatorcontrib><creatorcontrib>YE KAIJIE</creatorcontrib><creatorcontrib>ZHAI AIXIA</creatorcontrib><creatorcontrib>HE TAOJUN</creatorcontrib><creatorcontrib>CHEN XINGCHI</creatorcontrib><title>Novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as preparation method and application of B-cell immunodominance epitope peptide</title><description>The invention discloses a novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as a preparation method and application of the B-cell immunodominance epitope peptide, and belongs to the technical field of coronavirus detection. The method comprises the following steps: detecting and screening new coronavirus nucleocapsid protein specific IgG and IgM antibody positive serum samples by adopting an indirect ELISA method, overlapping the new coronavirus nucleocapsid protein to synthesize 68 short peptides, synthesizing 8 peptide libraries from the 68 short peptides, and screening to obtain an IgG and IgM immunodominance peptide library of the new coronavirus nucleocapsid protein. And finally, screening out the immunodominance epitope peptide with the sequence as shown in SEQ ID NO.66 again. The immunodominance epitope peptide does not contain unnecessary parts, can be used for serological detection and supplement of vaccine effect evaluation, and has great vaccine application va</description><subject>CHEMISTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjTEKwkAQRdNYiHqH8QApEiXaalCsUtmHYTPBgc3MsLuJV_KYRvQAFp8Hn8_7y-zV6EQezrkj74GHYRTtdGBBcQRknNQIjCxxR6A9OA0qOHEYI8joPKlDi9yBBU3EAhjh-XHNtECGAROrwEDpoR2gzDHz7L71bPzve50tevSRNj-usu31cq9vOZm2FA0dCaW2boqiKo_7sjqcdv9s3sRUWSw</recordid><startdate>20230623</startdate><enddate>20230623</enddate><creator>CHEN YING</creator><creator>ZHANG FANG</creator><creator>WU CHAO</creator><creator>WANG QINJIN</creator><creator>NING JIE</creator><creator>LI BIN</creator><creator>YE KAIJIE</creator><creator>ZHAI AIXIA</creator><creator>HE TAOJUN</creator><creator>CHEN XINGCHI</creator><scope>EVB</scope></search><sort><creationdate>20230623</creationdate><title>Novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as preparation method and application of B-cell immunodominance epitope peptide</title><author>CHEN YING ; ZHANG FANG ; WU CHAO ; WANG QINJIN ; NING JIE ; LI BIN ; YE KAIJIE ; ZHAI AIXIA ; HE TAOJUN ; CHEN XINGCHI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116284267A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>CHEMISTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>CHEN YING</creatorcontrib><creatorcontrib>ZHANG FANG</creatorcontrib><creatorcontrib>WU CHAO</creatorcontrib><creatorcontrib>WANG QINJIN</creatorcontrib><creatorcontrib>NING JIE</creatorcontrib><creatorcontrib>LI BIN</creatorcontrib><creatorcontrib>YE KAIJIE</creatorcontrib><creatorcontrib>ZHAI AIXIA</creatorcontrib><creatorcontrib>HE TAOJUN</creatorcontrib><creatorcontrib>CHEN XINGCHI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CHEN YING</au><au>ZHANG FANG</au><au>WU CHAO</au><au>WANG QINJIN</au><au>NING JIE</au><au>LI BIN</au><au>YE KAIJIE</au><au>ZHAI AIXIA</au><au>HE TAOJUN</au><au>CHEN XINGCHI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as preparation method and application of B-cell immunodominance epitope peptide</title><date>2023-06-23</date><risdate>2023</risdate><abstract>The invention discloses a novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as a preparation method and application of the B-cell immunodominance epitope peptide, and belongs to the technical field of coronavirus detection. The method comprises the following steps: detecting and screening new coronavirus nucleocapsid protein specific IgG and IgM antibody positive serum samples by adopting an indirect ELISA method, overlapping the new coronavirus nucleocapsid protein to synthesize 68 short peptides, synthesizing 8 peptide libraries from the 68 short peptides, and screening to obtain an IgG and IgM immunodominance peptide library of the new coronavirus nucleocapsid protein. And finally, screening out the immunodominance epitope peptide with the sequence as shown in SEQ ID NO.66 again. The immunodominance epitope peptide does not contain unnecessary parts, can be used for serological detection and supplement of vaccine effect evaluation, and has great vaccine application va</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN116284267A |
| source | esp@cenet |
| subjects | CHEMISTRY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS TESTING |
| title | Novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as preparation method and application of B-cell immunodominance epitope peptide |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T03%3A31%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=CHEN%20YING&rft.date=2023-06-23&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN116284267A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |