Novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as preparation method and application of B-cell immunodominance epitope peptide

The invention discloses a novel B-cell immunodominance epitope peptide of coronavirus nucleocapsid protein as well as a preparation method and application of the B-cell immunodominance epitope peptide, and belongs to the technical field of coronavirus detection. The method comprises the following steps: detecting and screening new coronavirus nucleocapsid protein specific IgG and IgM antibody positive serum samples by adopting an indirect ELISA method, overlapping the new coronavirus nucleocapsid protein to synthesize 68 short peptides, synthesizing 8 peptide libraries from the 68 short peptides, and screening to obtain an IgG and IgM immunodominance peptide library of the new coronavirus nucleocapsid protein. And finally, screening out the immunodominance epitope peptide with the sequence as shown in SEQ ID NO.66 again. The immunodominance epitope peptide does not contain unnecessary parts, can be used for serological detection and supplement of vaccine effect evaluation, and has great vaccine application va