Method for extracting hyodeoxycholic acid from pig bile

The invention relates to the technical field of extraction processes of hyodeoxycholic acid, in particular to a method for extracting hyodeoxycholic acid from pig bile, which comprises the following steps: alkalizing initial pig bile, siphoning, concentrating and distilling, standing and layering to...

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Hauptverfasser: ZENG HUI, XIAO KAIYONG, YAN FEI
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to the technical field of extraction processes of hyodeoxycholic acid, in particular to a method for extracting hyodeoxycholic acid from pig bile, which comprises the following steps: alkalizing initial pig bile, siphoning, concentrating and distilling, standing and layering to obtain a hyodeoxycholic acid stock solution, and crystallizing to obtain high-purity hyodeoxycholic acid. The specific purification method comprises the following steps: after a grey white hyodeoxycholic acid crude product is obtained, adding 10-12 times of ethyl acetate and 5-10% of activated carbon, heating and refluxing for 30-60 minutes, filtering, washing to be neutral, recovering the ethyl acetate until the volume is 1/8-1/4 of the original volume, cooling to room temperature, standing and crystallizing, and drying to obtain the high-purity hyodeoxycholic acid, the method effectively overcomes the defects of low product purity, high consumption of ethyl acetate and acetone and the like in the existing method