Method for extracting and purifying apigenin 7-O-glucoside from peony leaves

The invention belongs to the technical field of apigenin 7-O-glucoside providing methods, and particularly relates to a method for extracting and purifying apigenin 7-O-glucoside from peony leaves. The invention relates to a method for extracting and purifying apigenin 7-O-glucoside from peony leaves. The method comprises the following steps: S1, ethanol extraction; s2, extracting; s3, macroporous resin separation; s4, carrying out gel column separation; and S5, HPLC preparation and purification. Ethanol with high extraction efficiency is selected for extraction, extracts are separated through LX-38 macroporous resin and a Sephadex LH-20 gel column respectively, a standard substance and a sample are expanded under the same condition mainly according to thin-layer chromatography detection in the separation process, components with the same ratio shift value are combined, impurities are removed, a target compound is reserved, and the method is suitable for large-scale industrial production. And finally, apigeni