Low-cost HBV virus nucleic acid rapid detection method based on functionalized modified paper base

The invention discloses a low-cost hepatitis B virus (HBV) nucleic acid rapid detection method based on a functionalized modified paper base, which can realize rapid detection of HBV nucleic acid. The method comprises the following steps: (1) pre-amplifying HBV (Hepatitis B Virus) nucleic acid to obtain Texas red labeled nucleic acid; (2) capturing and enriching the Texas red labeled nucleic acid by adopting a functionally modified paper base; (3) carrying out signal detection; the functional modified paper base is modified with a fusion protein containing TR512 polypeptide. The protein functionalization modified paper base is easy to prepare, the nucleic acid detection time is short, and the detection cost is low. 本申请公开了一种基于功能化修饰纸基的低成本乙肝(HBV)病毒核酸快速检测方法，能实现对于HBV病毒核酸的快速检测。所述方法包括：(1)预扩增HBV病毒核酸，获得Texas red标记的核酸；(2)采用功能化修饰的纸基捕获并富集Texas red标记的核酸；(3)进行信号检测；所述功能化修饰的纸基上修饰有含有TR512多肽的融合蛋白。本申请中的蛋白功能化修饰纸基制备简单，核酸检测时间快速，检测成本低廉。