Nucleic acid aptamer capable of being specifically combined with African swine fever p30 protein and application of nucleic acid aptamer
The invention discloses a nucleic acid aptamer capable of being specifically combined with p30 protein of African swine fever ASFV and application of the nucleic acid aptamer, and belongs to the field of molecular immunity. The SELEX technology is utilized, ASFV p30 protein serves as a binding antig...
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| creator | LI CHENHUI ZHANG XINYU ZHAO CHENCHEN WANG WEIBO WANG YU YANG JIANYU LOU QUN HAO QINFANG LI KAI XU XIAOFANG ZHAO ZHONGLIANG SONG MINGXUAN WANG YANLING LIU ZUBIN YAN LEI YU JINGJING WANG JIAO DONG ZHAOYANG |
| description | The invention discloses a nucleic acid aptamer capable of being specifically combined with p30 protein of African swine fever ASFV and application of the nucleic acid aptamer, and belongs to the field of molecular immunity. The SELEX technology is utilized, ASFV p30 protein serves as a binding antigen, nucleic acid aptamers are screened out from a nucleic acid library, two nucleic acid aptamers with the highest binding force and good specificity are subsequently identified through aptamer secondary structure prediction, aptamer affinity determination, aptamer specificity detection and the like, and the core sequences of the two nucleic acid aptamers are shown as SEQ ID No: 1 and SEQ ID No: 2 respectively. The ASFV p30 protein specific nucleic acid aptamer screened and prepared by the invention has excellent specificity and affinity, and provides an important technical support for further research of an ASFV antigen detection technology.
本发明公开了一种可特异性结合非洲猪瘟ASFV的p30蛋白的核酸适配体及其用途，属于分子免疫领域。本发明利用SELEX技术，以ASFV p30蛋白作 |
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本发明公开了一种可特异性结合非洲猪瘟ASFV的p30蛋白的核酸适配体及其用途，属于分子免疫领域。本发明利用SELEX技术，以ASFV p30蛋白作</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230620&DB=EPODOC&CC=CN&NR=116265582A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230620&DB=EPODOC&CC=CN&NR=116265582A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LI CHENHUI</creatorcontrib><creatorcontrib>ZHANG XINYU</creatorcontrib><creatorcontrib>ZHAO CHENCHEN</creatorcontrib><creatorcontrib>WANG WEIBO</creatorcontrib><creatorcontrib>WANG YU</creatorcontrib><creatorcontrib>YANG JIANYU</creatorcontrib><creatorcontrib>LOU QUN</creatorcontrib><creatorcontrib>HAO QINFANG</creatorcontrib><creatorcontrib>LI KAI</creatorcontrib><creatorcontrib>XU XIAOFANG</creatorcontrib><creatorcontrib>ZHAO ZHONGLIANG</creatorcontrib><creatorcontrib>SONG MINGXUAN</creatorcontrib><creatorcontrib>WANG YANLING</creatorcontrib><creatorcontrib>LIU ZUBIN</creatorcontrib><creatorcontrib>YAN LEI</creatorcontrib><creatorcontrib>YU JINGJING</creatorcontrib><creatorcontrib>WANG JIAO</creatorcontrib><creatorcontrib>DONG ZHAOYANG</creatorcontrib><title>Nucleic acid aptamer capable of being specifically combined with African swine fever p30 protein and application of nucleic acid aptamer</title><description>The invention discloses a nucleic acid aptamer capable of being specifically combined with p30 protein of African swine fever ASFV and application of the nucleic acid aptamer, and belongs to the field of molecular immunity. The SELEX technology is utilized, ASFV p30 protein serves as a binding antigen, nucleic acid aptamers are screened out from a nucleic acid library, two nucleic acid aptamers with the highest binding force and good specificity are subsequently identified through aptamer secondary structure prediction, aptamer affinity determination, aptamer specificity detection and the like, and the core sequences of the two nucleic acid aptamers are shown as SEQ ID No: 1 and SEQ ID No: 2 respectively. The ASFV p30 protein specific nucleic acid aptamer screened and prepared by the invention has excellent specificity and affinity, and provides an important technical support for further research of an ASFV antigen detection technology.
本发明公开了一种可特异性结合非洲猪瘟ASFV的p30蛋白的核酸适配体及其用途，属于分子免疫领域。本发明利用SELEX技术，以ASFV p30蛋白作</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjTEKwkAQRdNYiHqH8QCCSYjYhqBYpbIPk8msDmx2h2Q1eAOP7QYsLaw-fN5_f5m86wdZFgIk6QA1YM8DECq2lsEbaFncDUZlEiOE1r6AfN-K4w4mCXcozRB7B-MUOzD8jHvN96CDD3EL6Gav2ggF8W52uh-f62Rh0I68-eYq2Z5P1-qyY_UNj4rEjkNT1Wl6yA5FcczK_B_mA0tAS2w</recordid><startdate>20230620</startdate><enddate>20230620</enddate><creator>LI CHENHUI</creator><creator>ZHANG XINYU</creator><creator>ZHAO CHENCHEN</creator><creator>WANG WEIBO</creator><creator>WANG YU</creator><creator>YANG JIANYU</creator><creator>LOU QUN</creator><creator>HAO QINFANG</creator><creator>LI KAI</creator><creator>XU XIAOFANG</creator><creator>ZHAO ZHONGLIANG</creator><creator>SONG MINGXUAN</creator><creator>WANG YANLING</creator><creator>LIU ZUBIN</creator><creator>YAN LEI</creator><creator>YU JINGJING</creator><creator>WANG JIAO</creator><creator>DONG ZHAOYANG</creator><scope>EVB</scope></search><sort><creationdate>20230620</creationdate><title>Nucleic acid aptamer capable of being specifically combined with African swine fever p30 protein and application of nucleic acid aptamer</title><author>LI CHENHUI ; ZHANG XINYU ; ZHAO CHENCHEN ; WANG WEIBO ; WANG YU ; YANG JIANYU ; LOU QUN ; HAO QINFANG ; LI KAI ; XU XIAOFANG ; ZHAO ZHONGLIANG ; SONG MINGXUAN ; WANG YANLING ; LIU ZUBIN ; YAN LEI ; YU JINGJING ; WANG JIAO ; DONG ZHAOYANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116265582A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LI CHENHUI</creatorcontrib><creatorcontrib>ZHANG XINYU</creatorcontrib><creatorcontrib>ZHAO CHENCHEN</creatorcontrib><creatorcontrib>WANG WEIBO</creatorcontrib><creatorcontrib>WANG YU</creatorcontrib><creatorcontrib>YANG JIANYU</creatorcontrib><creatorcontrib>LOU QUN</creatorcontrib><creatorcontrib>HAO QINFANG</creatorcontrib><creatorcontrib>LI KAI</creatorcontrib><creatorcontrib>XU XIAOFANG</creatorcontrib><creatorcontrib>ZHAO ZHONGLIANG</creatorcontrib><creatorcontrib>SONG MINGXUAN</creatorcontrib><creatorcontrib>WANG YANLING</creatorcontrib><creatorcontrib>LIU ZUBIN</creatorcontrib><creatorcontrib>YAN LEI</creatorcontrib><creatorcontrib>YU JINGJING</creatorcontrib><creatorcontrib>WANG JIAO</creatorcontrib><creatorcontrib>DONG ZHAOYANG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LI CHENHUI</au><au>ZHANG XINYU</au><au>ZHAO CHENCHEN</au><au>WANG WEIBO</au><au>WANG YU</au><au>YANG JIANYU</au><au>LOU QUN</au><au>HAO QINFANG</au><au>LI KAI</au><au>XU XIAOFANG</au><au>ZHAO ZHONGLIANG</au><au>SONG MINGXUAN</au><au>WANG YANLING</au><au>LIU ZUBIN</au><au>YAN LEI</au><au>YU JINGJING</au><au>WANG JIAO</au><au>DONG ZHAOYANG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Nucleic acid aptamer capable of being specifically combined with African swine fever p30 protein and application of nucleic acid aptamer</title><date>2023-06-20</date><risdate>2023</risdate><abstract>The invention discloses a nucleic acid aptamer capable of being specifically combined with p30 protein of African swine fever ASFV and application of the nucleic acid aptamer, and belongs to the field of molecular immunity. The SELEX technology is utilized, ASFV p30 protein serves as a binding antigen, nucleic acid aptamers are screened out from a nucleic acid library, two nucleic acid aptamers with the highest binding force and good specificity are subsequently identified through aptamer secondary structure prediction, aptamer affinity determination, aptamer specificity detection and the like, and the core sequences of the two nucleic acid aptamers are shown as SEQ ID No: 1 and SEQ ID No: 2 respectively. The ASFV p30 protein specific nucleic acid aptamer screened and prepared by the invention has excellent specificity and affinity, and provides an important technical support for further research of an ASFV antigen detection technology.
本发明公开了一种可特异性结合非洲猪瘟ASFV的p30蛋白的核酸适配体及其用途，属于分子免疫领域。本发明利用SELEX技术，以ASFV p30蛋白作</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TESTING VINEGAR WINE |
| title | Nucleic acid aptamer capable of being specifically combined with African swine fever p30 protein and application of nucleic acid aptamer |
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