Method and kit for detecting tigecycline-resistant high-virulence klebsiella pneumoniae based on CRISPR (clustered regularly interspaced short palindromic repeats)

The invention discloses a method and a kit for detecting tigecycline-resistant high-virulence klebsiella pneumoniae based on CRISPR (clustered regularly interspaced short palindromic repeats). The primer pair is designed and obtained according to target sites as shown in SEQ ID NO: 1-5. The crRNA pr...

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Bibliographische Detailangaben
Hauptverfasser:	XU CHUNYAN, DU XIANGDANG, LI CHENGLONG, WU YISHUAI, CHEN YINGJIE, YAO HONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a method and a kit for detecting tigecycline-resistant high-virulence klebsiella pneumoniae based on CRISPR (clustered regularly interspaced short palindromic repeats). The primer pair is designed and obtained according to target sites as shown in SEQ ID NO: 1-5. The crRNA probe is obtained by designing target sites as shown in SEQ ID NO: 6 to SEQ ID NO: 10. The invention discloses a kit for detecting tigecycline-resistant high-virulence klebsiella pneumoniae based on a CRISPR (clustered regularly interspaced short palindromic repeats) mode. The kit consists of freeze-drying reagents 1 and 2, buffer solutions 1 and 2, genome DNA (deoxyribonucleic acid) extraction reagents 1 and 2 and a purple flashlight. Treating the bacterial liquid by using a genome DNA extraction reagent; amplifying a target site gene through the primer pair, recombinase polymerase and dNTP (deoxyribonucleoside triphosphate); the invention relates to a method for rapidly and visually detecting tigecycline-resistant