Process and equipment for producing, separating and enriching microbial siderophore

The invention discloses a production, separation and enrichment process and equipment of a microbial siderophore, and the process comprises the following steps: (1) preparing an iron-deficient MSM culture medium, adjusting the pH value to 7.0, sterilizing, inoculating, and culturing in a sterile con...

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Bibliographische Detailangaben
Hauptverfasser:	ZHU LIHUA, LUO WENHAO, GUI YE, ZHANG RUICAI, ZHOU PENG, LIU YANG, YANG YAHENG, JANG JUN-WON, SUN MENGHAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY DISPOSAL OF SOLID WASTE ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROCESSES USING MICROORGANISMS RECLAMATION OF CONTAMINATED SOIL RECLAMATION OF CONTAMINED SOIL SOIL SPIRITS TRANSPORTING TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a production, separation and enrichment process and equipment of a microbial siderophore, and the process comprises the following steps: (1) preparing an iron-deficient MSM culture medium, adjusting the pH value to 7.0, sterilizing, inoculating, and culturing in a sterile constant-temperature shaking incubator for 96-120 hours to obtain a seed solution; (2) production and fermentation: stirring the iron-deficient MSM culture medium and the seed solution, enabling microorganisms to fully utilize nutrient substances of the culture medium, keeping the temperature of a culture room at 30 DEG C, and fermenting for 96-120 hours; (3) separation: centrifuging to separate thalli from the culture solution to obtain a supernatant rich in siderophores; and (4) enrichment: enabling the supernate to enter an enrichment layer through a pipeline, adsorbing the siderophore in the supernate by utilizing the high surface area characteristic of a loading material (charcoal or porous resin), and circularly