Extraction and purification method and application of fusobacterium nucleatum outer membrane vesicle

The invention discloses an extraction and purification method and application of outer membrane vesicles with fusobacterium nucleatum, and belongs to the technical field of biology. The method comprises the following steps: firstly, carrying out crude extraction on the outer membrane vesicles by utilizing an ultracentrifugation method to obtain crude extracted outer membrane vesicles, then preparing an iodixanol density gradient solution, and purifying the crude extracted outer membrane vesicles by utilizing a density gradient method to finally obtain the purified outer membrane vesicles. According to the method disclosed by the invention, an ultracentrifugation technology and an iodixanol density gradient centrifugation technology are combined, so that the problems that the extraction amount of the fusobacterium nucleatum outer membrane vesicles is small and toxic impurities remain are effectively solved. Cell experiment results show that the purified fusobacterium nucleatum outer membrane vesicle has low to