CRP camel source single-domain antibody as well as preparation method and application thereof

The invention discloses a CRP (C-reactive protein) camel source single-domain antibody as well as a preparation method and application thereof, and relates to the field of biological medicines, alpaca leukocytes immunized with a CRP antigen are enriched, and then single cell screening and sequencing are performed. The method comprises the following steps: analyzing a sequencing structure, constructing a camel source CRP antibody recombinant plasmid, cloning and converting an expression related antibody in eukaryotic cells, screening to obtain a monoclonal antibody with human CRP resistance, cloning and expressing a VHH structural domain of the monoclonal antibody, and pairing and screening to obtain the CRP-VHH monoclonal antibody with antibody activity. The antibody can be applied to an ELISA kit, a chemiluminiscence kit and a latex kit, has the same function as the existing kit, and also has the application in preparation of anti-tumor drugs. 本发明公开了一种CRP驼源单域抗体及其制备方法和应用，涉及生物医药领域，通过免疫CRP抗原的羊驼白细胞进行富集，然后进行单细胞筛选