Method for determining etomidate in blood and application

The invention relates to the technical field of analysis and detection, in particular to a method for determining etomidate in blood and application. The method comprises the following steps: pre-treating a blood sample by adopting a protein precipitation method to obtain supernate; then detecting the content of etomidate in the supernate by using a high performance liquid chromatography-mass spectrometry technology, wherein the chromatographic conditions in the high performance liquid chromatography-mass spectrometry technology are as follows: the model of a chromatographic column is an amino acid analysis column; a mobile phase A is 0.09-0.11 M ammonium formate water and contains 0.18% v/v to 0.22% v/v formic acid; a mobile phase B: acetonitrile containing 0.18% v/v-0. 22% v/v formic acid; the flow rate is 0.59 to 0.61 mL/min; the column temperature is 35-38 DEG C; and eluting in a gradient mode. The linear range of the detection method is 1-200ng/mL, the detection line is 1ng, and the method has the advant