Specific primer of molecular marker of Mongolian sheep gene B4GALNT2 and application of specific primer

The invention belongs to the field of molecular biology, and particularly relates to a specific primer of a molecular marker of a Mongolian sheep gene B4GALNT2 and application of the specific primer. According to the specific primer of the molecular marker of the Mongolian sheep gene B4GALNT2, the p...

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Hauptverfasser: CHENG ZIXUAN, WANG JIANGUO, ZHU YONG, LIU YONGBIN, TONG BIN, GUO XIN, YANG MINGTAO, CAO ZIWEI, HE MEI, LI NA, SHAO GUANGLONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of molecular biology, and particularly relates to a specific primer of a molecular marker of a Mongolian sheep gene B4GALNT2 and application of the specific primer. According to the specific primer of the molecular marker of the Mongolian sheep gene B4GALNT2, the primer sequences are shown as SEQ ID NO. 1 and SEQ ID NO. 2. According to the invention, a specific primer is designed to detect whether mutation of a g.25929793G/T site exists in an area from 25929578 bp to 25930324 bp of a No.7 intron of a B4GALNT2 gene in a Mongolian sheep genome, so as to compare the polymorphism of the B4GALNT2 gene in a Mongolian sheep variety. The mongolian sheep breeding is assisted by utilizing the polymorphism of the B4GALNT2 gene at the g.25929793G/T site, the lambing number of the mongolian sheep is increased, and the method can be used as an effective method for assisting in improving the multifetal traits of the mongolian sheep. 本发明属于分子生物学领域,具体涉及蒙古羊基因B4GALNT2的分子标记的特异性引物及其应用。蒙古羊基因B4GALN