Sphingomonas paucimobilis CRISPRi (clustered regularly interspaced short palindromic repeats i) system as well as construction method and application thereof

The invention discloses a sphingomonas paucimobilis CRISPRi system and a construction method and application thereof, and belongs to the technical field of genetic engineering, the construction method comprises the following steps: 1) screening a vector capable of being stably replicated and expressed in sphingomonas paucimobilis; (2) replacing the replicon fragment of the plasmid plv-dCas9-sgRNA with the replicon fragment of the vector to obtain a recombinant plasmid pBBR1-dCas9-sgRNA, namely a CRISPRi (clustered regularly interspaced short palindromic repeats i) system; and 3) determining a target gene, designing sgRNA, and verifying the inhibition efficiency of the CRISPRi system. The CRISPRi system comprises an expression vector carrying an sgRNA expression cassette and a dCas9 gene, and the vector carries chloramphenicol resistance. The CRISPRi system which is successfully constructed by the invention is transformed into sphingomonas paucimobilis by an electrotransformation method, so that genetic manipu