Time-resolved fluorescent microspheres as well as preparation method and application thereof

The invention relates to a time-resolved fluorescent microsphere as well as a preparation method and application thereof, the preparation method comprises the following steps: (1) performing emulsion polymerization reaction on styrene and carboxyl functional monomers by using a soap-free emulsion po...

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Bibliographische Detailangaben
Hauptverfasser:	GONG PISHENG, YANG CHENGFENG, ZHAO GUANGYAO, ZHOU SHIXIONG, WEI WENJING, SU DANDAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	ADHESIVES CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON DYES INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE MEASURING METALLURGY MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS NATURAL RESINS ORGANIC MACROMOLECULAR COMPOUNDS PAINTS PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS POLISHES TESTING THEIR PREPARATION OR CHEMICAL WORKING-UP THEIR RELEVANT APPARATUS TRANSPORTING
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Beschreibung
Zusammenfassung:	The invention relates to a time-resolved fluorescent microsphere as well as a preparation method and application thereof, the preparation method comprises the following steps: (1) performing emulsion polymerization reaction on styrene and carboxyl functional monomers by using a soap-free emulsion polymerization method to obtain a seed emulsion of carboxyl polystyrene microspheres; and (2) embedding the europium complex into the carboxyl polystyrene microspheres through a swelling method to obtain the time-resolved fluorescent microspheres. The preparation method disclosed by the invention is simple to operate, and the obtained time-resolved fluorescent microspheres are uniform and controllable in size, regular in surface, relatively high in fluorescence intensity and stable in performance. After the time-resolved fluorescent microsphere is coupled with the antibody, the time-resolved fluorescent microsphere has the characteristics of rapidness, simplicity, convenience, low cost, intuitive detection result, go