Targeted fully humanized CD22 chimeric antigen receptor and application thereof

The invention belongs to the technical field of cellular immune engineering, and particularly relates to a CD22-targeted chimeric antigen receptor and application thereof. The protein comprises an extracellular domain, a hinge region, a transmembrane region and an intracellular signal domain, and the extracellular domain comprises an amino acid sequence shown as Sequence NO.1 or a functional variant of the amino acid sequence. ScFv in the chimeric antigen receptor targeting CD22 provided by the invention is a brand-new humanized antibody sequence, the immunogenicity of a mouse antibody is avoided, obvious anti-tumor activity is shown after the chimeric antigen receptor is combined with CD22 on tumor cells, and a single-chain antibody of an extracellular antigen binding region belongs to a full human source, so that the chimeric antigen receptor has relatively high safety. 本发明属于细胞免疫工程技术领域，具体涉及一种靶向CD22的嵌合抗原受体及其应用。其包括胞外结构域、铰链区和跨膜区及胞内信号域，所述胞外结构域包括如SequenceNO.1所示的氨基酸序列或其功能性变体。本发明提供的靶向CD22的嵌合抗原受体中的ScFv是全新的人源抗体序列，避免