Fluorescent compound as well as preparation method and application thereof
The invention discloses a fluorescent compound as well as a preparation method and application of the fluorescent compound. The fluorescent compound is ER-Green or ER-Green-proRed. The ER-Green disclosed by the invention can specifically target the cell endoplasmic reticulum and emit bright green fl...
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| creator | SHI YILONG HAN SHOUFA ZOU XIAOXUE |
| description | The invention discloses a fluorescent compound as well as a preparation method and application of the fluorescent compound. The fluorescent compound is ER-Green or ER-Green-proRed. The ER-Green disclosed by the invention can specifically target the cell endoplasmic reticulum and emit bright green fluorescence, and when the cells are fixed by paraformaldehyde, the ER-Green is still retained in the cell endoplasmic reticulum; the ER-Green-proRed firstly specifically targets the endoplasmic reticulum of a cell and emits green fluorescence, and when endoplasmic reticulum autophagy occurs, the ER-Green-proRed enters an acid lysosome along with the endoplasmic reticulum and emits an acid-responsive red fluorescence signal, so that imaging of the endoplasmic reticulum autophagy process is realized.
本发明公开了一种荧光化合物及其制备方法和应用，该荧光化合物为ER-Green或ER-Green-proRed。本发明中的ER-Green可以特异性靶向细胞内质网并发出明亮的绿色荧光，当用多聚甲醛固定细胞时，ER-Green仍然保留于细胞内质网中；本发明中的ER-Green-proRed首先特异性靶向细胞内质网，发出绿色荧光，当发生内质网自噬时，伴随着内质网进入酸性溶酶体，发出酸响应的红色荧光信号，从而实现对内质网自噬过程的成像。 |
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本发明公开了一种荧光化合物及其制备方法和应用，该荧光化合物为ER-Green或ER-Green-proRed。本发明中的ER-Green可以特异性靶向细胞内质网并发出明亮的绿色荧光，当用多聚甲醛固定细胞时，ER-Green仍然保留于细胞内质网中；本发明中的ER-Green-proRed首先特异性靶向细胞内质网，发出绿色荧光，当发生内质网自噬时，伴随着内质网进入酸性溶酶体，发出酸响应的红色荧光信号，从而实现对内质网自噬过程的成像。</description><language>chi ; eng</language><subject>ADHESIVES ; CHEMISTRY ; DYES ; HETEROCYCLIC COMPOUNDS ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE ; MEASURING ; METALLURGY ; MISCELLANEOUS APPLICATIONS OF MATERIALS ; MISCELLANEOUS COMPOSITIONS ; NATURAL RESINS ; ORGANIC CHEMISTRY ; PAINTS ; PHYSICS ; POLISHES ; TESTING</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230606&DB=EPODOC&CC=CN&NR=116217486A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76294</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230606&DB=EPODOC&CC=CN&NR=116217486A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SHI YILONG</creatorcontrib><creatorcontrib>HAN SHOUFA</creatorcontrib><creatorcontrib>ZOU XIAOXUE</creatorcontrib><title>Fluorescent compound as well as preparation method and application thereof</title><description>The invention discloses a fluorescent compound as well as a preparation method and application of the fluorescent compound. The fluorescent compound is ER-Green or ER-Green-proRed. The ER-Green disclosed by the invention can specifically target the cell endoplasmic reticulum and emit bright green fluorescence, and when the cells are fixed by paraformaldehyde, the ER-Green is still retained in the cell endoplasmic reticulum; the ER-Green-proRed firstly specifically targets the endoplasmic reticulum of a cell and emits green fluorescence, and when endoplasmic reticulum autophagy occurs, the ER-Green-proRed enters an acid lysosome along with the endoplasmic reticulum and emits an acid-responsive red fluorescence signal, so that imaging of the endoplasmic reticulum autophagy process is realized.
本发明公开了一种荧光化合物及其制备方法和应用，该荧光化合物为ER-Green或ER-Green-proRed。本发明中的ER-Green可以特异性靶向细胞内质网并发出明亮的绿色荧光，当用多聚甲醛固定细胞时，ER-Green仍然保留于细胞内质网中；本发明中的ER-Green-proRed首先特异性靶向细胞内质网，发出绿色荧光，当发生内质网自噬时，伴随着内质网进入酸性溶酶体，发出酸响应的红色荧光信号，从而实现对内质网自噬过程的成像。</description><subject>ADHESIVES</subject><subject>CHEMISTRY</subject><subject>DYES</subject><subject>HETEROCYCLIC COMPOUNDS</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MISCELLANEOUS APPLICATIONS OF MATERIALS</subject><subject>MISCELLANEOUS COMPOSITIONS</subject><subject>NATURAL RESINS</subject><subject>ORGANIC CHEMISTRY</subject><subject>PAINTS</subject><subject>PHYSICS</subject><subject>POLISHES</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZPByyynNL0otTk7NK1FIzs8tyC_NS1FILFYoT83JAdEFRakFiUWJJZn5eQq5qSUZ-UBZkIqCgpzMZIhwSUZqUWp-Gg8Da1piTnEqL5TmZlB0cw1x9tBNLciPTy0uSATakVoS7-xnaGhmZGhuYmHmaEyMGgBVFzV-</recordid><startdate>20230606</startdate><enddate>20230606</enddate><creator>SHI YILONG</creator><creator>HAN SHOUFA</creator><creator>ZOU XIAOXUE</creator><scope>EVB</scope></search><sort><creationdate>20230606</creationdate><title>Fluorescent compound as well as preparation method and application thereof</title><author>SHI YILONG ; HAN SHOUFA ; ZOU XIAOXUE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116217486A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>ADHESIVES</topic><topic>CHEMISTRY</topic><topic>DYES</topic><topic>HETEROCYCLIC COMPOUNDS</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MISCELLANEOUS APPLICATIONS OF MATERIALS</topic><topic>MISCELLANEOUS COMPOSITIONS</topic><topic>NATURAL RESINS</topic><topic>ORGANIC CHEMISTRY</topic><topic>PAINTS</topic><topic>PHYSICS</topic><topic>POLISHES</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>SHI YILONG</creatorcontrib><creatorcontrib>HAN SHOUFA</creatorcontrib><creatorcontrib>ZOU XIAOXUE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SHI YILONG</au><au>HAN SHOUFA</au><au>ZOU XIAOXUE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Fluorescent compound as well as preparation method and application thereof</title><date>2023-06-06</date><risdate>2023</risdate><abstract>The invention discloses a fluorescent compound as well as a preparation method and application of the fluorescent compound. The fluorescent compound is ER-Green or ER-Green-proRed. The ER-Green disclosed by the invention can specifically target the cell endoplasmic reticulum and emit bright green fluorescence, and when the cells are fixed by paraformaldehyde, the ER-Green is still retained in the cell endoplasmic reticulum; the ER-Green-proRed firstly specifically targets the endoplasmic reticulum of a cell and emits green fluorescence, and when endoplasmic reticulum autophagy occurs, the ER-Green-proRed enters an acid lysosome along with the endoplasmic reticulum and emits an acid-responsive red fluorescence signal, so that imaging of the endoplasmic reticulum autophagy process is realized.
本发明公开了一种荧光化合物及其制备方法和应用，该荧光化合物为ER-Green或ER-Green-proRed。本发明中的ER-Green可以特异性靶向细胞内质网并发出明亮的绿色荧光，当用多聚甲醛固定细胞时，ER-Green仍然保留于细胞内质网中；本发明中的ER-Green-proRed首先特异性靶向细胞内质网，发出绿色荧光，当发生内质网自噬时，伴随着内质网进入酸性溶酶体，发出酸响应的红色荧光信号，从而实现对内质网自噬过程的成像。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | ADHESIVES CHEMISTRY DYES HETEROCYCLIC COMPOUNDS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE MEASURING METALLURGY MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS NATURAL RESINS ORGANIC CHEMISTRY PAINTS PHYSICS POLISHES TESTING |
| title | Fluorescent compound as well as preparation method and application thereof |
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