Fluorescent compound as well as preparation method and application thereof

The invention discloses a fluorescent compound as well as a preparation method and application of the fluorescent compound. The fluorescent compound is ER-Green or ER-Green-proRed. The ER-Green disclosed by the invention can specifically target the cell endoplasmic reticulum and emit bright green fluorescence, and when the cells are fixed by paraformaldehyde, the ER-Green is still retained in the cell endoplasmic reticulum; the ER-Green-proRed firstly specifically targets the endoplasmic reticulum of a cell and emits green fluorescence, and when endoplasmic reticulum autophagy occurs, the ER-Green-proRed enters an acid lysosome along with the endoplasmic reticulum and emits an acid-responsive red fluorescence signal, so that imaging of the endoplasmic reticulum autophagy process is realized. 本发明公开了一种荧光化合物及其制备方法和应用，该荧光化合物为ER-Green或ER-Green-proRed。本发明中的ER-Green可以特异性靶向细胞内质网并发出明亮的绿色荧光，当用多聚甲醛固定细胞时，ER-Green仍然保留于细胞内质网中；本发明中的ER-Green-proRed首先特异性靶向细胞内质网，发出绿色荧光，当发生内质网自噬时，伴随着内质网进入酸性溶酶体，发出酸响应的红色荧光信号，从而实现对内质网自噬过程的成像。