Primer probe combination for detecting eastern equine encephalomyelitis virus and application thereof

The invention discloses a primer probe combination for detecting eastern equine encephalomyelitis virus and application of the primer probe combination. The invention firstly discloses the primer probe composition for detecting the eastern equine encephalomyelitis virus, the primer probe composition comprises a specific tailed primer pair, a universal primer pair and an ultra-short probe, and the sequences are shown as SEQ ID NO.1-5. The invention also discloses a kit containing the primer probe composition, and a method for detecting the eastern equine encephalomyelitis virus established based on the primer probe composition. The method is sensitive and specific, and can quickly, accurately and quantitatively detect whether a sample to be detected is infected with the eastern equine encephalomyelitis virus or not. 本发明公开检测东部马脑脊髓炎病毒的引物探针组合及其应用。本发明首先公开了用于检测东部马脑脊髓炎病毒的引物探针组合物，包括特异性加尾引物对、通用引物对和超短探针，序列如SEQ ID NO.1-5所示。本发明还公开含有所述引物探针组合物的试剂盒，以及基于所述引物探针组合物建立的检测东部马脑脊髓炎病毒的方法。本发明的方法灵敏、特异，可以快速、准确、定量的检测出待测样本是否感染了东部马脑脊髓炎病毒。