Trichoderma reesei recombinant strain as well as preparation method and application thereof

The invention provides a trichoderma reesei recombinant strain as well as a preparation method and application thereof, the trichoderma reesei recombinant strain is trichoderma reesei TrEP-4, and the trichoderma reesei TrEP-4 is trichoderma reesei capable of overexpressing chaperonin. The trichoderma reesei recombinant strain TrEP-4 provided by the invention can be used for over-expression of chaperonin. Therefore, when the recombinant strain is used for producing the cellulase, the cellulase can be efficiently secreted and expressed under the condition of promoting correct folding and secretion of protein, and the production cost of the acid cellulase produced by the trichoderma reesei is reduced. 本发明提供了一种里氏木霉重组菌株、其制备方法及其应用，该里氏木霉重组菌株为里氏木霉TrEP-4，里氏木霉TrEP-4为可过表达伴侣蛋白的里氏木霉。本申请的里氏木霉重组菌株TrEP-4可过表达伴侣蛋白。因而，利用该重组菌株进行纤维素酶的生产，能够在促进蛋白质的正确折叠及分泌的情况下，得以高效分泌表达纤维素酶，降低里氏木霉产酸性纤维素酶的生产成本。