Preparation of conductive carbon nanospheres and calibration application of conductive carbon nanospheres in scanning electron microscope

The invention discloses preparation of conductive carbon nanospheres and calibration application of the conductive carbon nanospheres in a scanning electron microscope. The invention provides the preparation method of the conductive carbon nanosphere and the application of the conductive carbon nanosphere in the aspect of scanning electron microscope calibration. According to the invention, oxidation-auto-polymerization of dopamine is utilized to form a compact polydopamine layer on the surface of prepared spherical silicon dioxide, and the conductive carbon nanospheres are prepared through a subsequent heat treatment mode. The preparation steps are simple, the cost is relatively low, the repeatability and stability are relatively good, and large-scale batch preparation on the laboratory level can be realized. In addition, the prepared carbon nanospheres are good in conductivity and uniform in spherical particle size distribution, and high-resolution imaging can be kept under high vacuum and strong electron b