Immunodetection method based on aggregation-induced emission microspheres and magnetic separation technology

The invention discloses an immunodetection method based on aggregation-induced emission microspheres and a magnetic separation technology. The method comprises the following steps: mixing an antibody-1 labeled aggregation-induced emission microsphere solution and an antibody-2 labeled magnetic microsphere solution, adding an antigen-containing solution to be detected, measuring a fluorescence signal value, and obtaining the concentration of the antigen in the solution to be detected according to a standard curve. According to the invention, a luminous microsphere filled with aggregation-induced emission (AIE) molecules modified by carboxylic acid is used as a signal output element, is modified by a recognition antibody and then is compounded with a magnetic microsphere carrier modified by a corresponding antibody, and selective recognition of a marker is realized by using a double-antibody sandwich method or a competition method; a corresponding relation is formed between the measurement of the intensity of a