PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability, coding gene and engineering bacterium
The invention discloses a PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability, a coding gene and an engineering bacterium, the PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability is named as IsPETase S92P/D157A-cSP, the amino acid sequence of the IsPETase S92P/D157A...
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| creator | YIN QINGDIAN QI WEI SU RONGXIN YOU SHENGPING |
| description | The invention discloses a PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability, a coding gene and an engineering bacterium, the PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability is named as IsPETase S92P/D157A-cSP, the amino acid sequence of the IsPETase S92P/D157A-cSP is shown as SEQ ID NO.1, the melting temperature of the IsPETase S92P/D157A-cSP is measured to be greatly increased by a differential scanning fluorescence method, and the thermal stability of the IsPETase-cSP mutant enzyme is remarkably enhanced. When the IsPETase S92P/D157A-cSP is used for degrading the PET film, the degradation effect is obviously improved.
本发明公开了一种具有高热稳定性的PET水解酶IsPETase-cSP突变酶及编码基因及工程菌，一种具有高热稳定性的PET水解酶IsPETase-cSP突变酶，命名为IsPETaseS92P/D157A-cSP，所述IsPETaseS92P/D157A-cSP的氨基酸序列如SEQ ID NO.1所示，差式扫描荧光法测得其熔融温度大幅提升，热稳定性显著增强。使用IsPETaseS92P/D157A-cSP降解PET膜，降解效果显著提高。 |
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本发明公开了一种具有高热稳定性的PET水解酶IsPETase-cSP突变酶及编码基因及工程菌，一种具有高热稳定性的PET水解酶IsPETase-cSP突变酶，命名为IsPETaseS92P/D157A-cSP，所述IsPETaseS92P/D157A-cSP的氨基酸序列如SEQ ID NO.1所示，差式扫描荧光法测得其熔融温度大幅提升，热稳定性显著增强。使用IsPETaseS92P/D157A-cSP降解PET膜，降解效果显著提高。</description><language>chi ; eng</language><subject>AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS BASED THEREON ; COMPOSITIONS OF MACROMOLECULAR COMPOUNDS ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; GENERAL PROCESSES OF COMPOUNDING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC MACROMOLECULAR COMPOUNDS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; THEIR PREPARATION OR CHEMICAL WORKING-UP ; VINEGAR ; WINE ; WORKING-UP</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230530&DB=EPODOC&CC=CN&NR=116179508A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230530&DB=EPODOC&CC=CN&NR=116179508A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YIN QINGDIAN</creatorcontrib><creatorcontrib>QI WEI</creatorcontrib><creatorcontrib>SU RONGXIN</creatorcontrib><creatorcontrib>YOU SHENGPING</creatorcontrib><title>PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability, coding gene and engineering bacterium</title><description>The invention discloses a PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability, a coding gene and an engineering bacterium, the PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability is named as IsPETase S92P/D157A-cSP, the amino acid sequence of the IsPETase S92P/D157A-cSP is shown as SEQ ID NO.1, the melting temperature of the IsPETase S92P/D157A-cSP is measured to be greatly increased by a differential scanning fluorescence method, and the thermal stability of the IsPETase-cSP mutant enzyme is remarkably enhanced. When the IsPETase S92P/D157A-cSP is used for degrading the PET film, the degradation effect is obviously improved.
本发明公开了一种具有高热稳定性的PET水解酶IsPETase-cSP突变酶及编码基因及工程菌，一种具有高热稳定性的PET水解酶IsPETase-cSP突变酶，命名为IsPETaseS92P/D157A-cSP，所述IsPETaseS92P/D157A-cSP的氨基酸序列如SEQ ID NO.1所示，差式扫描荧光法测得其熔融温度大幅提升，热稳定性显著增强。使用IsPETaseS92P/D157A-cSP降解PET膜，降解效果显著提高。</description><subject>AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS BASED THEREON</subject><subject>COMPOSITIONS OF MACROMOLECULAR COMPOUNDS</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>GENERAL PROCESSES OF COMPOUNDING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC MACROMOLECULAR COMPOUNDS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>THEIR PREPARATION OR CHEMICAL WORKING-UP</subject><subject>VINEGAR</subject><subject>WINE</subject><subject>WORKING-UP</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNi80KwjAQhHvxIOo7rHcLFvHvKKWiFynYe9kmaxJM0tJskfr0RvABPM03w3zT5FkWFehR9q3FQHANsUdIxb0ENzB6BvLv0RG8DGvQRmlgTb1DC4GxMdbwuALRSuMVKPIE6GV0lPFE_XdsUHCkwc2TyQNtoMUvZ8nyXFT5JaWurSl0KKLOdX7Lsl22P27Xh9Pmn88HV5lAyw</recordid><startdate>20230530</startdate><enddate>20230530</enddate><creator>YIN QINGDIAN</creator><creator>QI WEI</creator><creator>SU RONGXIN</creator><creator>YOU SHENGPING</creator><scope>EVB</scope></search><sort><creationdate>20230530</creationdate><title>PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability, coding gene and engineering bacterium</title><author>YIN QINGDIAN ; QI WEI ; SU RONGXIN ; YOU SHENGPING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116179508A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS BASED THEREON</topic><topic>COMPOSITIONS OF MACROMOLECULAR COMPOUNDS</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>GENERAL PROCESSES OF COMPOUNDING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC MACROMOLECULAR COMPOUNDS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>THEIR PREPARATION OR CHEMICAL WORKING-UP</topic><topic>VINEGAR</topic><topic>WINE</topic><topic>WORKING-UP</topic><toplevel>online_resources</toplevel><creatorcontrib>YIN QINGDIAN</creatorcontrib><creatorcontrib>QI WEI</creatorcontrib><creatorcontrib>SU RONGXIN</creatorcontrib><creatorcontrib>YOU SHENGPING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YIN QINGDIAN</au><au>QI WEI</au><au>SU RONGXIN</au><au>YOU SHENGPING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability, coding gene and engineering bacterium</title><date>2023-05-30</date><risdate>2023</risdate><abstract>The invention discloses a PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability, a coding gene and an engineering bacterium, the PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability is named as IsPETase S92P/D157A-cSP, the amino acid sequence of the IsPETase S92P/D157A-cSP is shown as SEQ ID NO.1, the melting temperature of the IsPETase S92P/D157A-cSP is measured to be greatly increased by a differential scanning fluorescence method, and the thermal stability of the IsPETase-cSP mutant enzyme is remarkably enhanced. When the IsPETase S92P/D157A-cSP is used for degrading the PET film, the degradation effect is obviously improved.
本发明公开了一种具有高热稳定性的PET水解酶IsPETase-cSP突变酶及编码基因及工程菌，一种具有高热稳定性的PET水解酶IsPETase-cSP突变酶，命名为IsPETaseS92P/D157A-cSP，所述IsPETaseS92P/D157A-cSP的氨基酸序列如SEQ ID NO.1所示，差式扫描荧光法测得其熔融温度大幅提升，热稳定性显著增强。使用IsPETaseS92P/D157A-cSP降解PET膜，降解效果显著提高。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS OF MACROMOLECULAR COMPOUNDS COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY GENERAL PROCESSES OF COMPOUNDING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP VINEGAR WINE WORKING-UP |
| title | PET hydrolase IsPETase-cSP mutant enzyme with high thermal stability, coding gene and engineering bacterium |
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